Ceramide Enhances Acrosomal Exocytosis Triggered by Calcium and the Calcium Ionophore A23187 in Boar Spermatozoa
نویسندگان
چکیده
منابع مشابه
Calcium-dependent shortening of fibroblasts induced by the ionophore, A23187.
Attached, spread, chick heart fibroblasts were induced to shorten by treatment with the ionophore, A23187. The shortening resulted from the retraction of the leading lamellae and other major cell processes. The response was dependent on external calcium with a threshold close to, and a maximal effect at, physiological concentrations. The shortening was also induced in Colcemid-treated cells and...
متن کاملCalcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor.
The acrosome reaction of spermatozoa is a complex, calcium-dependent, regulated exocytosis. Fusion at multiple sites between the outer acrosomal membrane and the cell membrane causes the release of the acrosomal contents and the loss of the membranes surrounding the acrosome. However, very little is known about the molecules that mediate and regulate this unique fusion process. Here, we show th...
متن کاملParthenogenetic Activation of Porcine Oocytes by Calcium Ionophore A23187
This study was designated to clarify the influence of activation of porcine matured oocytes by calcium ionophore on in vitro development of the parthenotes. The follicular oocytes were matured, activated and cultured in North Carolina State University-23 (NCSU-23) medium supplemented with 10% porcine follicular fluid (pFF). The in vitro-matured oocytes were exposed to calcium ionophore at conce...
متن کاملArtificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa.
OBJECTIVE To evaluate the effect of artificial oocyte activation (AOA) on intracytoplasmic sperm injection (ICSI) cycles using surgically retrieved sperm. DESIGN Laboratory study. SETTING Fertility/assisted fertilization center. PATIENT(S) Couples undergoing surgical sperm retrieval for ICSI (n = 204). INTERVENTION(S) Application of calcium ionophore A23187 for AOA. MAIN OUTCOME MEASU...
متن کاملRegulation of acetylcholinesterase expression by calcium signaling during calcium ionophore A23187- and thapsigargin-induced apoptosis.
We have recently reported that acetylcholinesterase expression was induced during apoptosis in various cell types. In the current study we provide evidence to suggest that the induction of acetylcholinesterase expression during apoptosis is regulated by the mobilization of intracellular Ca(2+). During apoptosis, treatment of HeLa and MDA-MB-435s cells with the calcium ionophore A23187 resulted ...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2004
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.50.667